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Research Associate I - Recombinant Expression & Purification

San Diego - BioLegend Req ID JR-038814
Scientists wearing lab coats

BioLegend | About Us

At BioLegend, we are committed to providing researchers with the most comprehensive and cutting-edge high-quality reagents for life science research. Being part of Revvity, a global provider of health science solutions, technologies, expertise and services for life sciences and diagnostics, allows us to extend the mission of enabling our customers to do legendary discovery. Join us and make a difference!

Job Summary

The main job role of this position is recombinant protein expression from culture platforms such as E. Coli and performing protein purification using various preparative chromatography, formulating the recombinant proteins according to existing or newly developed procedures. Successful candidate as a protein purification associate plays an integral role in the success of recombinant protein development and manufacturing. He/she will be actively supporting scientists or team members in managing, and troubleshooting any processes including cross-functional product development teams within the organization.

Essential Functions

Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

  • Perform E. Coli culture and protein expression
  • Perform buffer and medium preparation for E. Coli culture
  • Perform protein purification methods on gravity or AKTA HPLC/ FPLC using affinity, ion exchange, hydrophobic, reverse-phase, and size exclusion chromatography.
  • Perform protein modification procedures such as His tag removal, biotinylation, and other activities for protein characterization such as UV spectrophotometry absorbance, quantification, and purity measurement.
  • Follow protocols and document protein purification production batch records to comply with ISO 13485.
  • Support optimizing processing in new product protein refolding/ purification for successful and smooth technical transfer
  • Effectively communicate and collaborate with project team members from multiple disciplines.
  • Other projects or responsibilities as may be required.

iACT Competencies

  • innovate – Be better, keep improving, be more efficient, creative, and be in the forefront of development
  • Aspire – Have desire, purpose, and ambition challenging ourselves to push the limits and reach new heights
  • Collaborate – Foster teamwork, common goals, selflessness, communication and mutual support
  • Transform – Adapt, learn, re-invent and change for future development and growth

Minimum Qualifications Education and Experience

  • Bachelor's Degree in Biology, Biochemistry, Chemistry, or other related area.
  • 6+ months of biotechnology industry experience in protein or antibody purification by various chromatography methods

Preferred Qualifications – Education and Experience

  • B.S. with 1+ year experience in the Biotechnology industry and quality system knowledge including ISO 13485.
  • Experience in protein product production including protein expression and purification protein
  • Experience in protein or antibody purification and modification.
  • Experience in process optimization for production scale-up

The base salary range for this full-time position is $26.44 - 31.25/hr. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training. Your recruiter can share more about the specific salary range for your preferred location during the hiring process. 

Please note that base pay is only one part of our total compensation package and is determined within a range. This range allows for the successful candidate to have an opportunity to progress within the position and develop at our company. This base pay range does not take into account bonuses, equity, or other benefits which may be applicable and are dependent on the level and position offered.

Work Environment & Physical Demands

Lab

While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job.

While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.

What do we offer?

We provide competitive and comprehensive benefits to our employees. Below are some highlights of our benefits:

  • Medical, Dental, and Vision Insurance Options
  • Life and Disability Insurance  
  • Paid Time-Off
  • Parental Benefits
  • 401k with Company Match
  • Employee Stock Purchase Plan

Learn more about Revvity’s benefits by visiting our Why Revvity page.

*For benefit-eligible roles only. Part-time and temporary roles may not be eligible for all benefits listed. Please reach out to your recruiter for more information.

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